Piezoelectric Immunosensor for SARS-Associated Coronavirus in Sputum
Identifieur interne : 005542 ( Main/Exploration ); précédent : 005541; suivant : 005543Piezoelectric Immunosensor for SARS-Associated Coronavirus in Sputum
Auteurs : Boli Zuo [République populaire de Chine] ; Shanmao Li [République populaire de Chine] ; Zhao Guo [République populaire de Chine] ; Jinfang Zhang [République populaire de Chine] ; Chuanzhi Chen [République populaire de Chine]Source :
- Analytical Chemistry [ 0003-2700 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- immunologie : Virus du SRAS.
- virologie : Expectoration.
- Adsorption, Dosage immunologique, Humains, Propriétés de surface, Science des ultrasons, Techniques de biocapteur, Virus du SRAS.
English descriptors
- KwdEn :
- MESH :
- chemistry : SARS Virus.
- immunology : SARS Virus.
- instrumentation : Biosensing Techniques, Immunoassay.
- methods : Biosensing Techniques, Immunoassay.
- virology : Sputum.
- Adsorption, Humans, Surface Properties, Ultrasonics.
Abstract
A piezoelectric immunosensor was developed for the detection of SARS-associated coronavirus (SARS-CoV) in sputum in the gas phase. Horse polyclonal antibody against SARS-CoV was bound onto the PZ crystal surface in an ordered orientation through protein A. The antigen sample was atomized into aerosol by an ultrasonator, by which the antibody on the crystal could specifically adsorb SARS antigen and the changed mass of crystal would lead a frequency shift. A frequency counter was employed to record the admittance frequency, and the plot of changed frequency was displayed on the computer. Under the optimized conditions, the frequency shifts were linearly dependent on antigen concentration in the range of 0.6−4 μg/mL. The device has good reproducibility (could be reused 100 times without detectable loss of activity), stability (the immunosensor was stable for more than two months when stored over silica gel blue at 4−6 °C), short analyzing time (less than 2 min), and specificity.
Url:
DOI: 10.1021/ac035367b
Affiliations:
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Chem.</title><idno type="ISSN">0003-2700</idno><idno type="eISSN">1520-6882</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2004</date><date type="published">2004</date><biblScope unit="vol">76</biblScope><biblScope unit="issue">13</biblScope><biblScope unit="page" from="3536">3536</biblScope><biblScope unit="page" to="3540">3540</biblScope></imprint><idno type="ISSN">0003-2700</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-2700</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adsorption</term><term>Biosensing Techniques (instrumentation)</term><term>Biosensing Techniques (methods)</term><term>Humans</term><term>Immunoassay (instrumentation)</term><term>Immunoassay (methods)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (immunology)</term><term>Sputum (virology)</term><term>Surface Properties</term><term>Ultrasonics</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adsorption</term><term>Dosage immunologique ()</term><term>Dosage immunologique (instrumentation)</term><term>Expectoration (virologie)</term><term>Humains</term><term>Propriétés de surface</term><term>Science des ultrasons</term><term>Techniques de biocapteur ()</term><term>Techniques de biocapteur (instrumentation)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term><term>Immunoassay</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>Immunoassay</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Expectoration</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Sputum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adsorption</term><term>Humans</term><term>Surface Properties</term><term>Ultrasonics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adsorption</term><term>Dosage immunologique</term><term>Humains</term><term>Propriétés de surface</term><term>Science des ultrasons</term><term>Techniques de biocapteur</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">A piezoelectric immunosensor was developed for the detection of SARS-associated coronavirus (SARS-CoV) in sputum in the gas phase. Horse polyclonal antibody against SARS-CoV was bound onto the PZ crystal surface in an ordered orientation through protein A. The antigen sample was atomized into aerosol by an ultrasonator, by which the antibody on the crystal could specifically adsorb SARS antigen and the changed mass of crystal would lead a frequency shift. A frequency counter was employed to record the admittance frequency, and the plot of changed frequency was displayed on the computer. Under the optimized conditions, the frequency shifts were linearly dependent on antigen concentration in the range of 0.6−4 μg/mL. The device has good reproducibility (could be reused 100 times without detectable loss of activity), stability (the immunosensor was stable for more than two months when stored over silica gel blue at 4−6 °C), short analyzing time (less than 2 min), and specificity.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zuo, Boli" sort="Zuo, Boli" uniqKey="Zuo B" first="Boli" last="Zuo">Boli Zuo</name></noRegion><name sortKey="Chen, Chuanzhi" sort="Chen, Chuanzhi" uniqKey="Chen C" first="Chuanzhi" last="Chen">Chuanzhi Chen</name><name sortKey="Guo, Zhao" sort="Guo, Zhao" uniqKey="Guo Z" first="Zhao" last="Guo">Zhao Guo</name><name sortKey="Li, Shanmao" sort="Li, Shanmao" uniqKey="Li S" first="Shanmao" last="Li">Shanmao Li</name><name sortKey="Zhang, Jinfang" sort="Zhang, Jinfang" uniqKey="Zhang J" first="Jinfang" last="Zhang">Jinfang Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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